Artificial reproduction of wild and pond-cultured common tench (Tinca tinca) under controlled conditions.

The common tench is an important thermophilic freshwater fish used for aquaculture diversification. This species is farmed in ponds as well as caught in the wild in many European and China. Artificial reproduction under controlled conditions is one of the most important part of modern aquaculture. In this study, two experiments were conducted in which the influence of hormonal agents (Ovopel and Ovaprim - experiment I) and the origin of the spawning stock (wild and pond-cultured - experiment II) on the success of reproduction in terms of ovulation rate (%), latency time (h), pseudo-gonadosomatic index (PGSI, %), hatching rate (%), abnormal larvae rate (%), and spawning efficiency coefficient (Se) were examined. While the wild fish could not reproduce in the absence of hormonal treatment with 0 % ovulation rate. In all tested hormonal treatment allowed to obtain high-quality eggs with hatching rates of over 83 %. Significant differences in latency time were observed among groups from different origins, such as differences in ovulation rate (79 % vs. 90 %, for pond-cultured and wild fish, respectively), PGSI (8.2 vs. 8.9), and hatching rate (63.8 % vs. 84.3 %), which showed significantly better quality in fish from the wild than those from pond culture. This means that when spawners of common tench of various origins are available, it is better to choose spawners from natural waters for artificial reproduction.

Artificial reproduction of the indoor-cultured brackish form of maraena whitefish (Coregonus maraena) under recirculated aquaculture system (RAS) conditions.

Maraena whitefish (Coregonus maraena) is a promising coldwater aquaculture species, especially in terms of meat quality. This versatile whitefish species, which inhabits both fresh and brackish waters, including the Baltic Sea, offers promising prospects for aquaculture breeding and cultivation. However, the main problem is the lack of availability of spawners of this species. Therefore, in this study, an attempt was made to raise the spawning stock under controlled conditions and then artificially reproduce the fish to obtain high-quality gametes and then larvae. The conducted research showed that successful culture of maraena whitefish spawners is possible under recirculating aquaculture system (RAS) conditions. The application of proper nutrition and manipulation of environmental conditions allowed the breeding of sexually mature whitefish spawners in less than two years in captivity (meat weight 476 g). Through stimulation of environmental conditions (temperature and photoperiod), it was possible to stimulate the final maturation of gametes of the F1 generation of cultured fish. The use of Ovaprim hormonal stimulation induced 100% of females to ovulate within 10 days of injection, compared to 60% of females in the control group. Additionally, the other examined parameters, such as the pseudogonado-somatic index (PGSI: 11.46% vs. 6.80%), fertilization rate (94.2% vs. 60.1%), embryo survival rate (92.6% vs. 41.1%) and embryo survival rate to the eyed-egg-stage (90.0% vs. 28.0), were significantly higher in the hormonally induced group, finally two times and three times, respectively. The mortality rate of spawners, after spawning and within three months of artificial spawning, was extremely low. The results obtained from the study demonstrate the feasibility of culturing the spawning stock of maraena whitefish in captivity and successfully inducing artificial spawning.

Growth Priorities of the Four Riverine Cyprinids during Early Ontogeny.

Growth patterns during early development of four species of riverine cyprinids, common dace (Leuciscus leuciscus L.), ide (Leuciscus idus L.), chub (Squalius cephalus L.) and common barbel (Barbus barbus L.), over 30 days of rearing, were analyzed. The study period covered three successive phases of larval development (preflexion, flexion, postflexion) and part of the juvenile development. The growth analysis carried out in the present study shows that significant allometric growth occurred mainly in preferentially localized body parts (head and tail) and was also associated with an increase in body height. The replacement of temporary structures (e.g., fin fold absorption) and the appearance of definitive structures around 21 mm TL (beginning of the juvenile period) corresponds to a stabilization of the relative growth of most parts of the body. This is probably due to the fact that the studied species had completed the most important part of the remodeling process (metamorphosis) and had achieved a body shape that allows juveniles to colonize faster water habitats. The results also indicate that individuals of species that are less developmentally advanced at the time of hatching (common barbel, chub) show greater initial dynamics of change in growth rate than individuals of species whose individuals are more advanced in ontogeny (common dace, ide). In addition, the small differences observed in growth parameters between successive periods analyzed, supported by the results of previous studies on these species, probably reflect the lack of abrupt changes in the behavior and habitat of the species studied.

Evaluation of Chemical Elements, Lipid Profiles, Nutritional Indices and Health Risk Assessment of European Eel (Anguilla anguilla L.).

The concentrations of ten elements (K, Na, Ca, Mg, Zn, Fe, Hg, Cu, Mn, and Cd) and fatty acids were analyzed in muscles of the European eel (Anguilla anguilla Linnaeus, 1758). The eels were caught in freshwater lakes connected with the Sawica River (north-eastern Poland). On this basis, it was determined whether the consumption of the fish is beneficial and safe for the health of the consumer. The results showed that the metal concentrations followed this order: K > Na > Ca > Mg > Zn > Fe > Hg > Cu > Mn > Cd. The fatty acids gave rise to the following sequence: MUFAs > SFAs > n-3 PUFAs > n-6 PUFAs. The target hazard quotient (THQ) value was below 1.0. The hazard quotient for the benefit-risk ratio HQEFA (0.39) also was below one, indicating that the intake of the recommended dose of EPA + DHA (250 mg/day) and the intake of mercury (0.415 mg/kg) for a person weighing 70 kg does not pose an obvious risk for human health. The lipid quality indices were OFA: 24.69, DFA: 74.36, AI: 0.55, and TI: 0.41. Based on the above statements, the consumption of eel meat is safe from a health point of view. However, the levels of toxic metals in the muscles of eels and their environment should continue to be monitored, as eels occupy a high position in the food chain.

Sex steroids in diploid and triploid gibel carp (Carassius gibelio) of both sexes in different phases of the reproductive cycle.

The changes in concentrations of 17ß-estradiol (E2), testosterone (T), 11-ketotestosterone (11-KT) in the gonads and plasma of diploid and triploid gibel carp (Carassius gibelio) from the Siemianowka Reservoir, the Vistula River drainage were quantified and compared using an enzyme immunoassay. The phase of gonad maturity was based on histological analysis and the gonadosomatic index. All fish had properly developed gonads in the pre-spawning, spawning and late spawning phases of the reproductive cycle in the fish collected in April, June and October, respectively. Diploid and triploid females and males did not differ in mean GSI in all reproductive phases. In October, ovaries of most females contained vitellogenic oocytes, testes had large numbers of spermatozoa. The hormone concentrations in both tissues depended mainly on the reproductive phase, but not on ploidy. The patterns of changes in E2 concentrations was similar in females and males. In the pre-spawning phase, diploid and triploid females showed differences in the concentrations of 11-KT in gonads and plasma, and T in plasma. Diploid males differed in pattern of T concentrations in gonads and plasma, while all males showed a similar pattern of 11-KT plasma concentrations. Spermatozoa observed in triploid males suggest that they participate in reproduction. Gibel carps, regardless of ploidy, had an extended period of reproduction, which makes this invasive species a potentially greater threat to native ichthyofauna. The sex androgen concentrations that differed between 2 n and 3 n females could be physiological factors potentially contributing to the coexistence of gynogenetic C. gibelio females and sexual diploids.

Total Mercury and Fatty Acids in Selected Fish Species on the Polish Market: A Risk to Human Health.

The muscles of lake trout (Salvelinus namaycush Walbaum, 1792), crucian carp (Carassius carassius Linnaeus, 1758), flounder (Platichthys flesus Linnaeus, 1758), gilthead seabream (Sparus aurata Linnaeus, 1758), mackerel (Scomber scombrus Linnaeus, 1758) and tench (Tinca tinca Linnaeus, 1758) were examined. The total mercury (THg) was processed using the Milestone DMA-80 and the fatty acids were analyzed using the 7890A Agilent Technologies chromatograph. The THg content in analyzed fish ranged from 0.024 (lake trout) to 0.092 mg/kg wet weight (gilthead seabream). The muscles of fish examined had lower amounts of SFAs, and n-3 and n-6 PUFAs than MUFAs. The ratio of n-3/n-6 was higher in muscles of mackerel than other fish (p < 0.05). Due to the fact that both the THQ and HI are below 1, the tested fish are safe for the consumer from a nutritional point of view. Similarly, fatty acid indices indicate the safe consumption of selected fish species, and the daily consumption of the recommended dose of EPA + DHA (250 mg/day) and the concentration of mercury in fish calculations showed a hazard quotient for the benefit−risk ratio HQEFA below 1, suggesting that the intake of EPA + DHA poses no evident risk to human health. The ratio was calculated for a person weighing 60 kg. Therefore, it is important to monitor the fish, not only bought in the store, but also caught in various aquatic environments.

Comparison of different methods of wild vimba bream Vimba vimba (L.) spawning under controlled conditions.

This paper describes a study in which different types of the reproduction of wild vimba bream (Vimba vimba) under controlled conditions were compared. Reproduction was performed by using three methods: spontaneous (natural spawning- NS groups): without hormones but with nests, semi-artificial (S groups) with hormones and nests, and artificial (A groups) with hormones and manual stripping of gametes. In groups S and A, different types of hormonal stimulation were tested: common carp pituitary homogenate (CPH), human chorionic gonadotropin (hCG), and commercial pellets containing mammalian GnRHa and dopamine antagonist-metoclopramide (Ovopel). The number of spawning fish, i.e., spermiating males and ovulating females, were compared depending on the type of breeding and the stimulant used. The lowest number of finally matured fish: spermiating males (67 %) and ovulating females (0 %) were noted in the spontaneously reproduced group. The best method of reproducing wild vimba bream under controlled conditions was the semi-artificial approach with hormonal stimulation and spawning nests, where ovulation rates in hormonally treated groups were between 43 % and 71 %, vs 26-57 % in artificial spawning groups. No ovulation observe in control groups in S and A types of reproduction. The highest embryo survival to the eyed-egg-stage was noted in group A-hCG (average 88.2). A higher value of spawning effectiveness (Se) with the use of the same hormones was obtained for groups S (0.374-0.574) than for groups A (0.252-0.493). In both cases, the highest Se was recorded for Ovopel stimulated fish and the lowest in hCG stimulated groups.

Influence of the source of spawners' origin on oocyte maturity stages and suitability for artificial reproduction of wild pikeperch (Sander lucioperca) females during spawning season.

Pikeperch is one of the major new fish species in freshwater aquaculture. Artificial reproduction is difficult in this species, and many aspects require further study. These include synchronization of female maturation, latency time, gamete quality and spawner survival rate. For these reasons, many fish farmers must constantly replenish their spawning pikeperch stock, often with wild fish. Data on the suitability for the reproduction of wild fish obtained in several lakes during the reproduction period were collected in this study. The findings indicated that larger fish (> 1.5 kg BW) and those obtained in larger and deeper lakes (> 12.0 m mean depth vs. < 5.0 m mean depth, respectively, for deep and shallow lakes) were more valuable to fish farmers during the spawning season when they were obtained because a higher percentage of suitability for artificial reproduction and a higher embryo survival rate was observed for them. The mean embryo survival rate was high and was 84.5 ± 3.9 % on the 3rd day after fertilization and 79.7 ± 3.4 % at hatching. Among the group of the smallest females (< 1.5 kg), the highest percentage of nonsynchronous oocyte maturation occurred. This result demonstrated the successful procedure of both artificial reproduction and a successful method for selecting females for spawning.

Effect of Application of Different Activation Media on Fertilization and Embryo Survival of Northern Pike, (Esox lucius) under Hatchery Conditions.

One of the finfish species that European and North American breeders are most interested in is the northern pike, Esox lucius. Artificial reproduction and the production of viable larvae has a huge impact on further culture. The quality of stripped gametes is highly variable. Therefore, it is important to use gametes with maximum efficiency, which has a direct impact on the amount of stocking material produced and therefore on the economics of production. The aim of this study was to compare northern pike fertilization efficiency, expressed as the survival rate of embryos until hatching. In the first experiment, the highest percentage of hatched embryos was observed in groups of eggs fertilized in a saline diluent prepared with deionized water (after reverse osmosis: group D), at 61.2% and 56.5% in the NaCl5-D and NaCl6-D groups, respectively. The highest percentage of hatched embryos in the second experiment was observed in the egg groups activated with Woynarovich solution (V) at 75.5% and 74.7% for V-D (D-deionized water) and V-T (T-tap water), respectively. In all cases, preparing the activation medium using T versus D water resulted in lower fertilization percentages and lower percentages of hatched larvae. At the same time, two variants (V and B1-Billard solution) were tested during mass spawning in three hatcheries using hatchery water (tap water). The results showed that repeatability was the highest when using activation medium B1.

Health benefits and potential applications of fucoidan (FCD) extracted from brown seaweeds in aquaculture: An updated review.

Nowadays, the application of immunomodulators in aquaculture has become of an urgent need because of high incidence of fish and shrimp diseases. For a long time, researchers have paid great interest to find suitable, relatively economical, and environmentally safe immunostimulant products to be used either as feed or water additives to boost immunity and increase the resistance of fish and shrimp against the challenging pathogens. Probiotics, prebiotics, synbiotics, phytobiotics, herbal extracts, microalgae, macroalgae, and essential oils have been extensively evaluated. Brown seaweeds (Phaeophyceae) are a large group of multi-cellular macroalgae that are widely distributed in marine aquatic environments. They are abundant in several bioactive sulfated polysaccharides known as fucoidan (FCD). Research studies demonstrated the beneficial functions of FCD in human medicine because of its immunomodulating, antioxidant, anti-allergic, antitumor, antiviral, anti-inflammatory, and hepatoprotective effects. In aquaculture, several researchers have tested the benefits and potential applications of FCD in aquafeed. This literature review provides an updated information and key references of research studies that focused principally on using FCD in aquaculture. Its effects on growth, intestinal health, antioxidant capacity, and immune responses of several finfish and shellfish species will be discussed. This review paper will also highlight the potential efficacy and mechanisms of FCD in the modulation of toxicity signs and increasing the resistance of fish and shrimp against bacterial and viral infections. Hence, this contribution will be valuable to maintain aquaculture sustainability and to improve the health and welfare of farmed fish and shrimp.

Effects of Bee Pollen on Growth Performance, Intestinal Microbiota and Histomorphometry in African Catfish.

This study aimed to determine the dietary effects of honeybee pollen (BP) on growth parameters, intestinal microbiota, hepatic histoarchitecture, and intestinal histomorphometry of African catfish Clarias gariepinus juveniles. The feeding experiment was carried out in a recirculating aquaculture system under controlled conditions for 21 days to achieve more than a 10-fold increase in weight in fish from the control group. Fish were fed well-balanced commercial feed without any supplements and served as a reference group (group C) and other diets enriched with varying BP levels as 1% (BP1), 2% (BP2), and 3% (BP3). Results showed a significant (p < 0.05) effect of the dietary BP not only on the growth parameters (such as final body weight: 5.0 g to 6.6−7.5 g, weight gain: 0.23 g/d to 0.31−0.35 g/d, body length: 84.7 mm to 93.8−95.9 mm, and specific growth rate: 11.7%/d to 13.1−13.7%/d, group C vs. experimental groups, respectively) but also on the development of beneficially important gut microbiota, such as lactic acid-producing bacteria. In BP-enriched groups, an average of 45% higher body weight gain was observed compared to those reared in the control group. The histological analysis showed that dietary BP may have a positive effect on the development of the intestinal tract and may enhance the absorption of nutrients with the potential ability to maintain a normal hepatic histoarchitecture of the treated African catfish. The results obtained suggest the optimum level of BP additive to feed for African catfish should be 1%.

Effects of GnRHa and hCG with or without dopamine receptor antagonists on the spawning efficiency of African catfish (Clarias gariepinus) reared in hatchery conditions.

Optimization of artificial reproduction is essential for minimizing genetic diversity, especially when fish are captured from their natural habitats and spawned in controlled conditions. In the present study, there was evaluation of the effects of gonadotropin-releasing hormone analogue (GnRHa) and human chorionic gonadotropin (hCG) with or without dopamine receptor antagonists such as domperidone (DOM) and metoclopramide (MET) on the spawning efficiency of African catfish (Clarias gariepinus) reared in captivity. The control group was intramuscularly (IM) injected with 1 mL of sterile saline solution. The fish specimens of the other six groups were injected IM with GnRHa or hCG, or in combination with either DOM or MET. None of the specimens had ovulations in the control group. There was the longest latency period in specimens treated with only GnRHa or hCG. There were the largest egg mass weight, fecundity, and hatchability (%) in specimens of the GnRHa + MET group. These findings indicate that GnRHa or hCG combined with dopamine receptor antagonists such as DOM and MET resulted in a marked enhancement of ovulation rate and increased the egg mass, fecundity, and hatchability of the treated C. gariepinus, and the values when there was inclusion of the MET treatment exceeded those when there was treatment with DOM.

Changes in Cadmium Concentration in Muscles, Ovaries, and Eggs of Silver European Eel (Anguilla anguilla) during Maturation under Controlled Conditions.

This study determined the contents of cadmium (Cd) in the muscles, ovaries, and eggs of silver female European eels. The analysis of cadmium content was performed on female European eels caught during commercial fishing in freshwater in Warmia and Mazury (Poland), and then subjected to artificial maturation and ovulation processing under controlled conditions. The content of cadmium (Cd) in the tissues was determined by flameless atomic spectrometry using an electrothermal atomizer. The analysis showed statistically significant differences between the cadmium content in the muscles, ovaries, and eggs (p < 0.05) of female European eels. The lowest cadmium content was found in the muscle tissue (0.0012 ± 0.0001 mg kg-1 wet weight) and the highest in eggs (after ovulation) (0.0038 ± 0.0007 mg kg-1 wet weight). Moreover, a relationship was found between the cadmium content in the muscle tissue and the ovaries (R = 0.673; p = 0.0117) in the same fish. The movement of cadmium from tissues to oocytes may indicate a significant problem concerning this heavy metal content in the reproduction of European eel.

Optimization of barbel (Barbus Barbus L.) fertilization and effects of ovarian fluid when there are controlled conditions for gamete activations.

Fertilization is one of the most important procedures in artificial reproduction and it directly affects the reproduction outcome. When there is optimization of fertilization, there can be a positive effect on subsequent reproductive processes and economic aspects of aquaculture. This study was conducted to determine time for which oocytes and sperm of barbel Barbus barbus retain fertilization capacity following placement in freshwater. Furthermore, the amount of ovarian fluid, excreted by fish during spawning with eggs (OFI; %) was determined, along with the chemical composition and effects on fertilization were determined. Gametes, ovarian fluid, and seminal plasma from barbel spawning specimens of the F4 generation were used to conduct the study. Ovarian fluid accounted for 14%-68% of contents of the mass released at spawning and post-spawning composition differed depending on whether hormonal treatments were utilized for control of reproduction. There was an association (R2 = 0.982; P = 0.000) between the pH of ovarian fluid and the barbel embryo survival rate. There was the greatest survival rate (>60 %) when the pH range of 7.9-8.4 and there was a lesser embryo viability when pH values were lesser or greater than values within this range (P <  0.05). The results from the study indicate that barbel eggs retain fertilization capacity longer (as long as 210 s) after activation by placement in fresh water than spermatozoa (about 30 s).

Comparison of responses to artificial spawning of ruffe (Gymnocephalus cernua) specimens captured from their natural habitat to those produced in cultured conditions.

Although ruffe (Gymnocephalus cernua) are widely distributed in Eurasia, in some regions (i.e., North America) ruffe are considered an invasive species. There have been no reports on artificial reproduction of this species. A study, therefore, was conducted to evaluate reproduction of ruffe with results of specimens captured from their natural habitat and cultured (F1 generation) specimens being compared. Spawning specimens from both stocks were treated with hormonal preparations: carp pituitary homogenate (CPH), Ovopel, Ovaprim and human chorionic gonadotropin (hCG) and results were analyzed by comparing response to the specimens of control groups. Spermiation outcomes and sperm motility of the captured and cultured ruffe were similar and with all hormonal treatments, there was a slightly greater sperm motility (55.6 %-57.1 %) in comparison to specimens of control groups (46.7 %-47.1 %). For captured specimens, there was no asynchronous development of oocytes, whereas in cultured specimens 32 % of females had asynchronous development of oocytes. The ovulation rate in specimens of all treated groups was 100 %, whereas specimens in the control groups did not reproductively mature and have ovulations. The latency time from time of hormonal treatments to initiation of reproductive functions depended on the spawning agent used and oocyte maturation stage and there was the shortest latency after using CPH and the longest with hCG treatment. The embryo survival and hatching rates varied with use of different hormonal preparations to induce reproduction: greatest hatching rates with hCG treatment (86.4 %-88.9 %), followed by Ovaprim (78.2 %-80.2 %) and least hatching rate with Ovopel and CPH treatments (66.0 %-67.1 % and 64.0 %-66.0 %, respectively).

Using Rutilus rutilus (L.) and Perca fluviatilis (L.) as Bioindicators of the Environmental Condition and Human Health: Lake Lanskie, Poland.

The aim of this study was to determine the mercury content and fatty acids profile in roach (Rutilus rutilus L.) and European perch (Perca fluviatilis L.) from Lake Lanskie (Poland). Mercury content was higher in the muscles than other organs in both species (p < 0.05). Mercury accumulates along the food chain of the lake's ecosystem. The value of the bioconcentration factor (BCF) indicated that Hg had accumulated in the highest amounts in muscles and in the other organs as follows: muscles > liver > gills > gonads. The metal pollution index (MPI) and target hazard quotient (THQ) were below 1, which means that these fish are safe for consumers. The values of HIS, GSI and FCF indicators show that both species of fish can be good indicators of water quality and food contamination. There were few differences between fatty acid content in the muscles of perch and roach. Contents of fatty acids having an undesirable dietary effect in humans (OFA-hypercholesterolemic fatty acids) were lower compared to hypocholesterolemic fatty acids (DFA, i.e., the desirable ones). In addition, the lipid quality indices AI and TI in the muscles of fish were at 0.40 and 0.22 (perch) and at 0.35 and 0.22 (roach), respectively. On this basis, it can be concluded that the flesh of the fish studied is beneficial from the health point of view.

Inhibiting the influence of ovarian fluid on spermatozoa activation and spermatozoa kinetic characteristics in the common barbel Barbus barbus.

As a result of evolution, various finfish species have developed different breeding strategies. However, there are some similarities, and one of them is the positive effect of ovarian fluid on spermatozoa. The opposite of this phenomenon was found in the common barbel (Barbus barbus). The present study analyzed the effect of ovarian fluid (OF), distilled water (DW) and Woynarovich solution (WS) on the motility, longevity and kinetics of barbel spermatozoa. These spermatozoa parameters were also evaluated with various dilutions of ovarian fluid (OF) in relation to distilled water [0:4 (Group OF 0%), 1:3 (Group OF 25%), 1:1 (Group OF 50%), 3:1 (Group OF 75%), 4:0 (Group OF 100%)] and spermatozoa reactivation after a 30 s (Group OFR30s 100%) treatment in ovarian fluid. The motility analysis was carried out using computer-assisted semen analysis (CASA). The negative interaction of ovarian fluid with spermatozoa motility in the same fish species was recorded for the first time. In pure ovarian fluid, the average spermatozoa motility (MOT) decreased significantly (1.40 ± 0.94%). The negative effect of ovarian fluid-to-spermatozoa motility was reversible, and after a 30 s treatment in ovarian fluid and later dilution with water, spermatozoa motility was reactivated (from 2.25 ± 0.53% vs 69.78 ± 6.02%). The use of Woynarovich solution as an activator of spermatozoa movement had a positive effect (P < 0.05) on spermatozoa movement longevity (motility up to 90 s) and the percentage of motile spermatozoa compared to distilled water (up to 45 s) and ovarian fluid (P < 0.05).

Comparison of artificial spawning effectiveness of hCG, CPH and GnRHa in combination with dopamine inhibitors in a wild strain of ide Leuciscus idus (L.) in hatchery conditions.

Optimization of artificial reproduction, by increasing the survival rate of embryos and hatching rate, is of major importance for reducing genetic diversity, especially in fish captured from their natural habitat that subsequently spawn in hatcheries. The artificial reproduction of ide, Leuciscus idus (L.) was conducted in controlled conditions. The spawning agents included: different doses of human chorionic gonadotropin (hCG) that was compared with common carp pituitary homogenate (CPH), Ovopel - a commercial agent for induction of spawning that contains mammalian gonadotropin releasing hormone analogue (mGnRHa) with dopamine antagonists (DA): metoclopramide (MET) and Ovaprim - a commercial agent containing salmon gonadotropin releasing hormone analogue (sGnRHa) and a dopamine antagonists (DA): domperidone (DOM). There were no ovulations in females from control groups. There were no differences between the ovulation rates (90 %) or embryo survival (> 92 %) and hatching rates (> 91 %) when there was administration of hCG doses between 500 and 1000 IU/kg. When there was comparison of different spawning agents, the ovulation rate was 100 % for all treated groups. There were the shortest and longest latency times to the time of ovulation after administration of CPH (26 h) and hCG (79 h), respectively. The greatest embryo survival (> 93 %) and hatching (> 91 %) rates occurred as a result of hCG administration with these values being slightly greater than when there was treatment with Ovaprim. The association between latency time and hatching rate indicated that when there was a slower final oocyte maturation (FOM) there were greater hatching rates.

Optimization of artificial insemination outcomes of African catfish (Clarias gariepinus) with differing hatchery conditions.

The African catfish is one of the most promising warm-freshwater species for aquaculture development in the upcoming years, although there are two primary limitations in its production: less-than-desirable survival rates and low larvae quality. In this study, a novel method of egg fertilization for this species was evaluated which has already been successfully utilized in the production of other finfish. The results indicate that dividing the semen designated for fertilization of eggs into smaller aliquots and adding it to the eggs at 30 s intervals (i.e., 0, 30 and 60 s), after activation of the eggs with water, increased the hatching rate percentage (97.1%) compared to control groups (87.9%) in which the entire portion of semen was added to eggs at the time of water addition for egg activation. Results with repeated evaluations in the field confirmed that eggs with greater biological quality were fertilized at a similar rate using the modified and control methods for fertilization, although when the eggs were of a lesser biological quality, the fertilization rate was greater using the modified methods than the control methods.

First report of the occurrence and different types of conjoined twins in common whitefish Coregonus maraena larvae originating from the Baltic Sea.

This paper describes for the first time various forms of conjoined twins in the common whitefish Coregonus maraena originating from the brackish waters of the Baltic Sea. The occurrence of conjoined twins was observed during the hatching of whitefish under controlled conditions. Two types of twins were identified: symmetric (parapagus) and asymmetric (parasitic form). The percentage of conjoined twins among the whitefish did not exceed 0.01% of the hatched larvae. The forms of twins observed in this study were similar to conjoined twins occurring among humans. One main type, parapagus, was identified in the group of symmetric twins with 3 subtypes: diprosopus parapagus, dicephalic parapagus and dithoracic parapagus. In its parasitic form, the parasitic twin occurred as a 'cyclops' or just as an eye growing out of the other twin's head. We conclude that fish, as well as their fertilised eggs, can be used as a model system to observe how such twins are joined in humans because of the similarity with conjoined twins occurring in humans. Because of internal fertilisation in fish, as well as the size and transparency of egg cells in these animals, it is possible to observe the process of fertilisation, cell division and formation of an embryo and subsequently of twins, as a model for humans.